Journal: Journal of Inflammation Research

Article Title: Supplementation of Glucosamine Selenium Ameliorates DSS-Induced Chronic Colitis in Mice via Affecting Gut Microbiota, Inhibiting Pyroptosis and Inactivating Chemokine Signaling Pathway

doi: 10.2147/JIR.S486751

Figure Lengend Snippet: GASe affects chemokine signaling pathway. ( A ) Volcano plots show upregulated and downregulated DEGs in the GSE87466 dataset and GSE53306 dataset. Red plots represent upregulated genes and blue ones represent downregulated genes. ( B ) A total of 114 co-DEGs at the intersection of GSE87466 and GSE53306 datasets. ( C and D ) KEGG enrichment analysis for 114 co-DEGs. ( E ) Western blot analysis reveals GASe inhibits the expression of CXCL1 and CXCL2 in chronic DSS colitis mice. *** p < 0.001, compared with the control group. ## p < 0.01 and ### p < 0.001, compared with the DSS group.

Article Snippet: To verify the effect of GASe on the chemokine pathway, the chemokine pathway antagonist reparixin (1 μM, Med-ChemExpress) was added to the DSS + GASe-DS cells.

Techniques: Western Blot, Expressing, Control

Journal: Journal of Inflammation Research

Article Title: Supplementation of Glucosamine Selenium Ameliorates DSS-Induced Chronic Colitis in Mice via Affecting Gut Microbiota, Inhibiting Pyroptosis and Inactivating Chemokine Signaling Pathway

doi: 10.2147/JIR.S486751

Figure Lengend Snippet: GASe-DS alleviates DSS-induced cell apoptosis and inflammatory response via inactivating chemokine signaling pathway. ( A ) The effects of series of GASe-DS content on cell viability. ( B ) 1% GASe-DS and reparixin increase cell viability, while ATI-2341 TFA decreases cell viability of DSS-treated NCM460 cells. ( C ) Flow cytometry shows that GASe-DS and reparixin treatment decrease cell apoptosis, while ATI-2341 TFA increases cell apoptosis. ( D ) Administration of GASe-DS and reparixin decrease the levels of IL-12, IL-6 and TNF-α. ATI-2341 TFA promotes the expression of IL-10, IL-6 and TNF-α. ( E ) Western blot analysis reveals that administration of GASe-DS and reparixin promote the expression of ZO-1, Occludin and claudin-1, and ATI-2341 TFA downregulates ZO-1, Occludin and claudin-1 in NCM460 cells. ( F ) Administration of GASe-DS and reparixin downregulate the expression of CXCL1 and CXCL2, which is reversed by ATI-2341 TFA in NCM460 cells. *** p < 0.001, compared with the control group. ### p < 0.001, compared with the DSS group. & p < 0.05, && p < 0.01, and &&& p < 0.001, compared with the DSS + GASe-DS group.

Article Snippet: To verify the effect of GASe on the chemokine pathway, the chemokine pathway antagonist reparixin (1 μM, Med-ChemExpress) was added to the DSS + GASe-DS cells.

Techniques: Flow Cytometry, Expressing, Western Blot, Control

Journal: iScience

Article Title: Caloric restriction protects from acute and chronic kidney injury by inhibiting monocyte recruitment

doi: 10.1016/j.isci.2025.113094

Figure Lengend Snippet: FMD reduces CCL2 and monocytes recruitment at 14 days after AA injection (A) Circulating CD45 + CD11b + myeloid cells changes (%) over baseline at 7 and 14 ( ad lib n = 10, FMD n = 7, Low Cal n = 8) days after AA injection. (B) Circulating CD45 + CD11b + myeloid cells changes (%) over baseline at 7, 14, 21, 28, and 34 ( ad lib n = 4, FMD n = 4) days after diet (FMD versus Ad lib ) without AA. (C) Serum CCL2 levels (pg/mL) at 0 and 14 days ( ad lib n = 18, FMD n = 12) after AA injection. (D) Serum CCL2 levels (pg/mL) at 0, 14 and 35 days after diet (FMD versus Ad lib ) without AA ( ad lib n = 4, FMD n = 4). (E) CCL2 mRNA levels in kidney and liver in ad lib and FMD mice 14 days after AA injection ( ad lib n = 6, FMD n = 8, ad lib liver n = 6, FMD liver n = 8). (F) Linear regression analysis of serum CCL2 levels and kidney CCL2 transcripts 14 days after AA injection. (G) Linear regression analyses between serum CCL2 levels and liver CCL2 transcripts 14 days after AA injection. (H and I) (H) Representative histogram and (I) quantification of CCR2 expression in spleen CD45 + CD11b + Ly6G − Ly6C hi pro-inflammatory monocytes; fluorescence was evaluated as gMFI (geometric mean fluorescent index) in ad lib vs. FMD mice after AA injection ( ad lib n = 5, FMD n = 5). Repeated measures ANOVA model was used to assess statistical significance at different time points (A, B, C, and D). Correlation and linear regression analyses were performed to measure association. t test was used to compare gMFI distributions between groups at the same time point (Panel I). Data are represented as mean ± SEM ○: p < 0.05 vs. baseline; ○○ p < 0.01 vs. baseline ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001 vs. ad lib at the same time point; ns, not significant.

Article Snippet: CCR2 chemokine receptor antagonist , Tocris Bioscience/Bio-Techne , 2517.

Techniques: Injection, Expressing, Fluorescence

Journal: Cells

Article Title: Male-Dominant Spinal Microglia Contribute to Neuropathic Pain by Producing CC-Chemokine Ligand 4 Following Peripheral Nerve Injury.

doi: 10.3390/cells14070484

Figure Lengend Snippet: Figure 3. Sexually dimorphic effect of CCR5 antagonist maraviroc in relieving neuropathic pain. Male and female mice were subjected to PSL, and maraviroc (20 nmol) was intrathecally (i.t.) administered once on day 7 after PSL. The 50% mechanical threshold on days 0 (pre) and 7 after PSL, and 3 and 24 h after i.t. administration of maraviroc in male and female mice were assessed employing the up–down method using the von Frey test (n = 6, two-way ANOVA followed by Tukey’s multiple comparison test, *** p < 0.001, ** p < 0.01 vs. Veh/cont, ## p < 0.01).

Article Snippet: A CC-chemokine receptor 5 (CCR5) antagonist (Maraviroc; Tocris Biosciences, Bristol, UK) was dissolved in dimethyl sulfoxide and diluted in sterile PBS for further use.

Techniques: Comparison